The Muse(TM) Cell Dispersal Reagent ー100 Tests (Part Number: MCH100107) is an enzymatic reagent that has been formulated to gently disaggregate the cells that have aggregated in clumps in order to obtain uniform suspensions for cell counting. Cell samples containing cell aggregates may not yield accurate or consistent results with the Muse Count & Viability Reagent alone due to differential staining in clumps. Additionally, cell aggregates may clog or result in coincident events, hampering the assay1-3. Cell lines such as the Chinese Hamster Ovary (CHO-K1) cell line that have been adapted to suspension culture tend to form cell aggregates4,5. The Muse Cell Dispersal Reagent is useful for disaggregating clumpy suspension cultures in combination with the Muse Count & Viability Reagents, and yields improved accuracy and consistency for count and viability data with the Muse Count & Viability Assay2. Data generated with the Muse Software module provides:
●Viable cell count (cells/mL)
●Total cell count (cells/mL)
●Percentage viability of sample
The Muse Cell Dispersal Reagent is optimized for use with the Guava(R) Muse(TM) Cell Analyzer, which makes sophisticated, fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse Cell Analyzer, intuitive software provides detailed or summary analysis of your cell sample in a few short steps.
For Research Use Only. Not for use in diagnostic procedures.
1. Muse(TM) Count & Viability User’s Guide, Document Part No. 4600-3373. Luminex, 2019.
2. Magallanes E, Helsel C, Liu B, Yokobata K. Validating the Guava(R) Viacount(TM)CDR(TM)Assay. Technical Note, Guava Technologies Inc, 2003.
3. Phi-Wilson J, Harvey J, Goix P, O’Neill R. A technology for the rapid acquisition of cell number and viability. Am Biotechnol Lab. May 2001:34-36.
4. Shen BP, Bodnar CA, Manuel J, Siegel AC, Levy GA. Comparison of Serum-Free Suspension Culture Media for the Growth and Production of a Protein-Producing CHO Suspension Line. Application Note. Industrial BioDevelopment Laboratory.
5. Hernandez C, Christie A, MacNorton S, Wilson S, et al. Optimization of Cell Counting Throughput for the SAFC Raw Materials Characterization Program. SAFC, Lenexa.