The Muse(TM) Count & Viability Kit 200X ー100 Tests (Part Number: MCH100104) was developed for absolute cell count and viability determination of difficult cell samples. It is designed to address the need for a compatible reagent for non-mammalian cell lines, such as SF-9 insect cells. These cells prefer significantly lower pH and higher osmolarity than mammalian cells for optimal viability. The reagent contains the same fluorescent DNA-binding dyes used in the Muse Count & Viability Kit, but in a highly concentrated formulation.
These dyes have differential permeability to viable and non-viable cells, and provide absolute cell count and viability data on cell suspensions from a variety of cultured cell lines. Both viable and non-viable cells are differentially stained based on their permeability to the DNA-binding dyes in the reagent. Data generated using the Guava(R) Muse(TM) Cell Analyzer with the Muse Software provides:
●Viable cell count (cells/mL)
●Total cell count (cells/mL)
●Percentage viability of sample
The Muse Count & Viability Kit 200X allows you to assess a greater variety of cell types on the Muse Cell Analyzer. You can also use it to prepare a custom formulation of a reagent in a buffer or medium compatible with your unique cell line. It can be added directly to the cell sample for counting, allowing direct and rapid determination of absolute cell count and viability with dilute cell samples, down to 1x104 cells/mL. It can also be used to prepare a more concentrated, custom formulation, which may reduce incubation time and optimize fluorescent signals for cell lines that stain less efficiently. The Muse Count & Viability Kit 200X allows for assessment of absolute cell count and viability of challenging cell samples rapidly, reliably, and conveniently.
For Research Use Only. Not for use in diagnostic procedures.