The Guava(R) Cytochrome c Kit ー100 Tests (Part Number: FCCH100110) assesses the loss of mitochondrial cytochrome c in cells that have been induced to undergo apoptosis. The kit includes an anti-cytochrome c-FITC antibody, an anti-IgG1-FITC isotype control, as well as optimized fixation, permeabilization, and blocking buffers to allow for the detection of cytochrome c using flow cytometry.
The kit may be used on a flow cytometer equipped with a 488 nm laser which detects FITC probes. The kit also uses specialized buffer sets to achieve selective permeabilization of mitochondria, while leaving the mitochondrial membrane intact. Viable and live cells will have higher levels of cytochrome c staining, while apoptotic cells, which have released cytochrome c from the mitochondria into the cytoplasm, will demonstrate reduced staining intensity when probed with an anti-cytochrome c-FITC antibody. Stained cells can then be analyzed by flow cytometry.
The kit includes all of the fluorescently-labeled antibodies and buffers necessary for cell preparation and analysis. This flow cytometry method, from staining to analysis, can be completed within three hours after sample induction.
Components:Anti-Cytochrome c-FITC, Anti-IgG1-FITC, 10X Permeabilization Buffer, 5X Fixation Buffer, 1X Blocking Buffer
For Research Use Only. Not for use in diagnostic procedures.